Abstract: INTRODUCTION: In Brazil, Mato Grosso (MT) has the highest number of hantavirus cardiopulmonary syndrome cases. Our study aimed to identify anti-hantavirus antibodies in the sera of patients from Sinop, MT, presenting with acute febrile illness. METHODS: A retrospective analysis of data for 198 sera samples assessed using enzyme-linked immunosorbent assay (ELISA) was conducted. RESULTS: Immunoglobulins G (IgGs) against the hantavirus nucleoprotein were found in 13.6% of the tested sera. No sample had immunoglobulin M (IgM) antibodies to hantavirus. Seropositivity occurred mainly in female residents in urban areas who worked around the household. CONCLUSIONS: Our findings suggest circulation of hantavirus in Sinop.

Avaliou-se a precisão do método FAMACHA© na identificação de fêmeas ovinas alimentadas com dois níveis de proteína bruta, infectadas naturalmente por Haemonchus contortus por meio do valor de hematócrito correspondente. Foram utilizadas 47 fêmeas ovinas das raças Santa Inês (n = 16), Texel (n = 16) e Ile de France (n = 15), com idades entre oito e 12 meses. Dentro de cada raça, os animais foram distribuídos em dois tratamentos, 12 ou 16% de proteína bruta total na dieta. Todos os animais foram vermifugados 30 dias antes das primeiras coletas de dados que foram feitas quinzenalmente, no período entre julho de 2005 e março de 2006. Avaliou-se individualmente a mucosa ocular de todos os animais em concordância com o preceituado pelo método FAMACHA©, bem como se obteve o valor do hematócrito de cada animal avaliado em laboratório. Obteve-se uma correlação de 1:0,7991 dos valores de hematócrito e a classificação pelo método FAMACHA© visando a identificação de animais acometidos por diferentes graus de anemia. O método mostrou-se eficiente na identificação dos animais que necessitam de tratamento antiparasitário constituindo-se como suporte na identificação de animais susceptíveis ao Haemonchus contortus.

The accuracy of the FAMACHA© method was evaluated on the identification of female sheep fed two levels of crude protein, naturally infected with Haemonchus contortus, by means of the correspondent hematocrit value. Forty-seven female sheep of the breeds Santa Inês (n = 16), Texel (n = 16) and Ile de France (n = 15) aged between eight and twelve months were assigned to two treatments, 12 or 16 % crude protein in the diet. All the animals were wormed thirty days before the first data collections, which were done fortnightly between July 2005 and March 2006. The color of the ocular conjunctiva was individually evaluated according to the precepts of the FAMACHA© method and the hematocrit value of each animal was obtained in laboratory. A correlation of 1:0.7991 was found between the hematocrit values and the classification given by the FAMACHA© method aiming to identify animals with different degrees of anemia. The method was efficient to identify animals to worm, thus representing a support in the identification of animals susceptible to Haemonchus contortus.

The incidence of estrus in nulliparous Santa Inês (n = 16), Texel (n = 16) and Ile de France (n = 15) ewes fed two levels of crude protein (CP, 12 or 16%) was monitored from July 2005 to December 2006, and seasonality in the Santa Inês breed in the south of Brazil was characterized. The solar radiation data were recorded daily, and samples of blood were collected biweekly for determination of the plasma concentration of progesterone in Santa Inês lambs at, on average, 11±1 months of age. The female Santa Inês lambs, in the experimental period, stayed among teaser rams with a powdered-dye-ink mixture placed on their chest to mark the females that accepted to be mounted. Santa Inês ewes did not manifest estrus in the first fortnight of November and in December 2005, nor in the last fortnight of December 2006. Estrus activity was not observed on any of the three breeds in October 2006. Breeds differed at the level of 12% CP. Santa Inês and Ile de France females did not differ as for the probability estrus manifestation and both presented higher probabilities then Texel. When the effect of 12 or 16% CP on each breed was evaluated separately, it was verified that levels of 12 or 16% of crude protein did not change the probability of estrus manifestation in any of the studied breeds. The concentration of plasma progesterone in Santa Inês ewes during the spring of 2005 and 2006 indicated that there is difference between 12% CP (0.68±1.32 ng/mL) and 16% CP (1.28±1.99 ng/mL) and between the years 2005 (0.39±0.78 ng/mL) and 2006 (1.47±2.08 ng/mL), demonstrating the anestrous seasonality of Santa Inês in South Brazil.

The objective of this work was to evaluate the influence of low-stress handling (LSH) on reactivity score and pregnancy rate during fixed-time artificial insemination (FTAI) for extensively raised Nellore cows. Multiparous cows (n = 126) were randomly allocated into two groups: G1 (n = 66) and G2, the control group (n = 60). Group G1 was subjected to LSH, in which the animals were handled in a calm and quiet manner, without loud noises or physical aggression, using the point of balance, respecting the flight zone and using flags to supplement handling. Group G2 was handled following the typical procedure, with yelling, kicking and the use of electric prods and sticks. On D0, D8 and D10, FTAI was performed in both groups. Reactivity was scored on D0, D8 and D10 at the squeeze chute, based on the tension score, breathing score, and bellowing score. Using the three criteria above, the reactivity scores were defined as follows: R1 (calm); R2 (slightly reactive); R3 (moderately reactive); R4 (reactive); and R5 (highly reactive). Thirty-five days after artificial insemination, pregnancy was determined using ultrasonography. There was no significant correlation between reactivity score and pregnancy rate in each group or between the pregnancy rates in both groups. However, there was a statistically significant difference between the G1 and G2 groups with regard to reactivity score (1.62±0.05 vs. 2.12±0.07). Low-stress handling influenced reactivity but did not affect pregnancy rate in extensively raised Nellore cows subjected to FTAI.

Foram utilizadas 80 ovelhas Santa Inês, com 47,49 ± 3,76 kg de peso vivo, cobertas com reprodutores Dorper e alimentadas com concentrado com 15,20; 22,48; 26,52 e 35,65% de proteína bruta (PB) no terço inicial de gestação. As ovelhas, durante o dia, permaneceram em pastagem de capim-aruana (Panicum maximum cv. Aruana). Ao nascimento, foram realizadas biópsias no músculo Semitendinosus esquerdo de cordeiros machos, para avaliar o número e o diâmetro das fibras musculares. Na fase de terminação, os cordeiros foram distribuídos em dois grupos e alimentados com uma dieta controle, contendo 16,18% de proteína bruta (PB) e 79,64% nutrientes digestíveis totais (NDT), sem grão de girassol; ou com uma dieta contendo 9,10% de grãos de girassol (15,03% de PB e 78,96% de NDT). Não houve efeito da alimentação materna sobre o peso dos cordeiros ao nascimento (4,82 kg), aos 30 dias de idade (9,69 kg) e ao desmame (15,38 kg). O sexo influenciou o peso dos cordeiros ao nascimento e ao desmame. O desempenho dos cordeiros na fase de terminação não foi influenciado pela dieta fornecida nessa fase, nem pelo sexo nem pelo manejo alimentar da mãe durante a gestação. Não houve diferença no número (2813,45) nem no diâmetro (13,16 μm) das fibras musculares ao nascimento. O teor de PB de dietas para ovelhas no terço inicial da gestação pode ser reduzido, pois essa redução não influencia no diâmetro e número de fibras musculares.

It was used 80 Santa Inês ewes with average body weight 47.79 ± 3.76 kg, mated with Dorper rams and fed concentrated diet with 15.20; 22.48; 26.52 and 35.65% crude protein (CP) on the initial third of pregnancy. Sheep were kept on aruana grass (Panicum maximum cv. Aruana) pasture during the day. At birth, biopsy on the left Semitendinosus muscle were done on male lambs to evaluate the number and diameter of muscular fibers. During finishing phase, lambs were distributed in two groups and fed control diet with 16.18% crude protein (CP) and 79.64% total digestible nutrients (TDN); without sunflower grain; or fed diet with 9.10% sunflower grain (15.03% CP and 78.96% TDN). There was no effect of maternal feeding on the weight of lambs at birth (4.82 kg), at 30 days of age (9.69 kg) and at weaning (15.38 kg). Sex affected weight of lambs from birth to weaning. Performance of the lambs during finishing phase was not affected by diet given during this phase, neither by the sex nor by maternal food management during pregnancy. There was no difference on number (2,813.45) neither on diameter (13.16 μm) of the muscle fibers on birth. Contents of crude proteins in diets for sheep at the initial third of pregnancy can be reduced because this reduction does not affect diameter and number of muscle fibers.